SPECIFICATIONS

Excitation Wavelength:
488 nm (CW Solid State Laser)

Emission Range:
>505 nm (band pass filter)

Lower Detection Limit:
80 pM for a single fluorescein (clearly visible migration boundary). The lower detection limit depends on fluorophore’s intrinsic quantum yield (Q), quenching, the number of fluorophores per molecule, and other intangibles.

Usable Range:
Four Decades above the lower detection limit for fluorescein. Upper limit can also be reduced by inner filter effect. Dyes with a lower Q, or additional absorbance from other sources, will diminish the range.

Linearity:
Signal is linear to within 1% over Useable Range.

Radial Resolution:
50 μm (limited by spot size)
Data can be acquired at 2 μm intervals

Scan Time:
~60 seconds typical (20 μm step size, averaging 5 intensities at each step at 30,000 rpm). All cells are scanned simultaneously. The scan time is rotor speed dependent.

Rotors, Cells and Centerpieces:
The fluorescence operating system can be used with any of the current rotors, cells, and centerpieces, including short column centerpieces.